Saturday, April 29, 2017

Mira4 assembly of 454 reads from SRA

I want to make an assembly of the Annona squamosa fruit transcriptome data from this paper ( They give in the paper a link to a web resource (, but the resource appears to now be defunct, so to get contigs reads, I will have to assemble the reads myself. The reads are from two different cultivars of Annona squamosa, so I'm going to assemble each cultivar separately first, and then if that works, I'll try a combined assembly.

MIRA is a nice, free, software package that can assemble 454 data. I've had success with it before, so that's what I'll use for this project too.